Heterologous Expression and Efficient Secretion of Chitosanase from Microbacterium sp. in Escherichia coli

Isolation, characterization and heterologous expression of a novel chitosanase from Janthinobacterium sp. strain 4239

BACKGROUND Chitosanases (EC 3.2.1.132) hydrolyze the polysaccharide chitosan, which is composed of partially acetylated beta-(1,4)-linked glucosamine residues. In nature, chitosanases are produced by a number of Gram-positive and Gram-negative bacteria, as well as by fungi, probably with the primary role of degrading chitosan from fungal and yeast cell walls for carbon metabolism. Chitosanases ...

Expression and efficient secretion of a functional chitinase from Chromobacterium violaceum in Escherichia coli

BACKGROUND Chromobacterium violaceum is a free-living β-proteobacterium found in tropical and subtropical regions. The genomic sequencing of C. violaceum ATCC 12472 has revealed many genes that underpin its adaptability to diverse ecosystems. Moreover, C. violaceum genes with potential applications in industry, medicine and agriculture have also been identified, such as those encoding chitinase...

Cloning and Expression of Cyclophilin from Platanus orientalis Pollens in Escherichia coli

Background: Allergy is a clinical disorder affecting the human population with wide geographical distribution. Platanus orientalis (P. orientalis) trees are planted in many countries and their pollen causes allergic reactions. Cyclophilin has recently been identified as one of the most important allergens of P. orientalis pollen. We aimed to clone and purify this allergen in Escherichia coli...

High-Yield Expression of Heterologous [FeFe] Hydrogenases in Escherichia coli

BACKGROUND The realization of hydrogenase-based technologies for renewable H(2) production is presently limited by the need for scalable and high-yielding methods to supply active hydrogenases and their required maturases. PRINCIPAL FINDINGS In this report, we describe an improved Escherichia coli-based expression system capable of producing 8-30 mg of purified, active [FeFe] hydrogenase per ...

Advances and challenges in recombinant protein expression in Escherichia coli